一、研究室概况
Laboratory Overview
研究室名称
Laboratory Name
耳鼻咽喉头颈外科研究室
Laboratory of Otolaryngology-Head and Neck Surgery
位置：前沿医学中心D5A栋A401、E4栋5楼、华西临床教学楼（新八教）6021
Location: Building D5A, A401 & Building E4, 5th Floor，Frontier Medical Center;Clinical Teaching Building (New Building 8), 6021, West China Hospital
联系方式：电话028 8542 1671，邮箱1006427545@qq.com
Contact Information: Phone: 028-8542 1671, Email: 1006427545@qq.com
研究方向检索词
Research Focus Keywords
肿瘤免疫学、肿瘤发生发展机理、类器官模型构建及应用、内耳基因治疗、耳鼻喉遗传疾病、耳鼻喉变态反应科学、大数据挖掘、喉移植、高分子材料、人工智能等
Tumor immunology, the mechanisms of tumorigenesis and development, the construction and application of organoid models, gene therapy for the inner ear, genetic diseases of the ear, nose, and throat, allergic reactions in the ear, nose, and throat, big data mining, laryngectomy, high molecular materials, artificial intelligence, etc.
研究方向（简要）
Research Focus(Brief Overview)
（1）肿瘤免疫治疗
Tumor immunotherapy
（2）肿瘤发生发展机理
The Mechanism of Tumor Development and Progression
（3）肿瘤新抗原鉴定及疫苗免疫
Identification of novel tumor antigens and vaccine-based immunotherapy.
（4）类器官模型构建及应用
Organoid Model Development and Applications
（5）内耳基因治疗
Gene Therapy for the Inner Ear
（6）中耳炎症发生发展机制
Mechanism of development of middle ear inflammation
（7）内耳发育及疾病模型
Inner Ear Development and Disease Models
（8）耳聋遗传
Hereditary Deafness
（9）鼻变态反应
Nasal Allergic Reaction
（10）上呼吸道炎症性疾病
Upper Respiratory Inflammatory Diseases
（11）咽喉病变相关材料研发
Development of materials related to throat disorders
（12）喉移植免疫排异及肿瘤复发
Larynx transplantation immune rejection and tumor recurrence
（13）大数据挖掘、人工智能模型
Big Data Mining, Artificial Intelligence Models
研究室简介
Laboratory Introduction
耳鼻咽喉头颈外科研究室依托学科建设方向成立，紧密围绕临床科学问题开展一系列深入研究。现有3处办公及实验场地，分别位于成都前沿医学中心E4栋5楼、D5A栋4楼，华西临床教学楼6楼，面积1000余平。现有PI 17名，在站博士后17名，数据分析师4名，实验技术员3名，科研助理6名，以及全日制硕士、博士研究生30余人。研究室主要围绕耳鼻咽喉头颈科学开展相关研究，拥有20个特色技术平台，研究内容覆盖多组学数据分析、模式动物构建、类器官培养、基因编辑、小分子靶向递送、药物筛选、疫苗研发等多个前沿技术领域。研究室目前承担了国家自然科学基金项目（重点/面上/青年）、科技部重点研发计划、四川省科技厅重点项目和四川大学华西医院学科卓越发展1•3•5工程项目等纵向项目百余项。研究室与加拿大多伦多大学玛格丽特公主医院、西班牙加泰罗尼亚生物工程研究所、德国埃森大学、新加坡国立大学、比利时根特大学、瑞典卡罗林斯卡大学、香港大学、香港中文大学、香港城市大学等多所国际著名高校、科研院所开展紧密合作。在头颈肿瘤、耳聋遗传、内耳发育、上呼吸道免疫、类器官模型、基因编辑、疫苗免疫、高分子生物材料、喉移植等多个领域进行了一系列深入研究，相关成果发表于Nature communication、Science Advance、Elife、npj Vaccine、Science Signaling and Translational Therapeutics、Journal of Allergy and Clinical Immunology、Allergy、Journal of Clinical Immunology、Ear and Hearing、Hearing Research等杂志上。
The Otolaryngology-Head and Neck Surgery Research Laboratory was established in alignment with the department‘s academic development focus, conducting in-depth research closely tied to clinical scientific questions. The laboratory operates across three locations: the 5th floor of Building E4 and the 4th floor of Building D5A at the Chengdu Frontier Medical Center, and the 6th floor of the West China Clinical Teaching Building. These facilities cover a total area of over 1,000 square meters. The research team consists of 17 Principal Investigators (PIs), 17 postdoctoral researchers, 4 data analysts, 3 laboratory technicians, 6 research assistants, and more than 30 full-time master’s and doctoral students. The laboratory focuses on various aspects of otolaryngology-head and neck science, supported by 20 specialized technical platforms. Key research areas include multi-omics data analysis, model organism development, organoid cultivation, gene editing, small-molecule targeted delivery, drug screening, and vaccine development. Currently, the laboratory is undertaking over 100 projects, including grants from the National Natural Science Foundation of China (Key/General/Young Investigator Programs), the Ministry of Science and Technology’s Key R&D Program, the Sichuan Provincial Science and Technology Department, and the "1•3•5" Excellence Development Project at West China Hospital, Sichuan University.
The laboratory collaborates closely with prestigious international institutions such as Princess Margaret Cancer Centre (University of Toronto, Canada), the Institute for Bioengineering of Catalonia (Spain), the University of Duisburg-Essen (Germany), the National University of Singapore, Ghent University (Belgium), Karolinska Institute (Sweden), the University of Hong Kong, the Chinese University of Hong Kong, and the City University of Hong Kong. Our extensive research spans head and neck oncology, hereditary deafness, inner ear development, upper respiratory immunity, organoid models, gene editing, vaccine immunology, polymer biomaterials, and laryngeal transplantation. Research findings have been published in leading journals such as Nature Communications, Science Advances, eLife, npj Vaccines, Science Signaling, Translational Therapeutics, Journal of Allergy and Clinical Immunology, Allergy, Journal of Clinical Immunology, Ear and Hearing, and Hearing Research.
耳鼻咽喉头颈外科研究室与罕见病研究院院长袁慧军教授建立紧密合作关系，联合成立耳鼻喉-罕见病联合实验室，围绕罕见病致病基因鉴定、多组学融合分析解析疾病基因调控机制、大人群遗传变异数据库建设、罕见病靶向药物及诊治新技术开发、罕见病精准诊治智能决策支持系统开发等方面开展多项合作课题。
The Research Laboratory of Otorhinolaryngology-Head and Neck Surgery and the Director of the Institute of Rare Diseases, Professor Yuan Huijun, have established a close cooperative relationship and jointly established the Otorhinolaryngology-Rare Diseases Joint Laboratory to conduct a number of cooperative research projects on gene identification for rare diseases, multi-omics integration analysis to elucidate the gene regulatory mechanisms of the diseases, construction of a large population genetic variation database, development of targeted drugs and new diagnostic and treatment technologies for rare diseases, and development of intelligent decision support systems for precise diagnosis and treatment of rare diseases.
二、特色平台/设备/技术
Unique Platforms/Equipment/Technologies
特色平台
Unique Platform
1）肿瘤、喉移植等模式动物平台；
Tumor, larynx transplantation, etc. model animal platforms;
2）多组学数据分析平台；
Multi-omics data analysis platform;
3）专病临床生物样本管理平台；
Disease-Specific Clinical Biological Sample Management Platform;
4）类器官构建及药物筛选创新平台；
Platform for constructing organoids and drug screening innovation;
5）免疫肽库构建及候选抗原鉴定平台；
Platform for constructing immune peptide libraries and identifying candidate antigens;
6）疫苗免疫研究平台；
Vaccine Immunization Research Platform;
7）内耳发育与再生模式动物平台；
Inner Ear Development and Regeneration Model Animal Platform;
8）动物听觉功能测听实验平台；
Animal Hearing Function Audiometry Test Platform;
9）膜片钳电生理实验平台；
patch-clamp electrophysiology experimental platform;
10）耳组织病理学实验平台；
Histopathological Experimental Platform for Ear Tissue;
11）耳聋基因诊断治疗技术开发平台；
Platform for the Development of Gene Diagnosis and Therapy for Deafness;
12）药物靶向递送平台；
Drug-targeted delivery platform;
13）CRISPR/Cas9基因编辑技术靶点筛选平台；
CRISPR/Cas9 Gene Editing Technology Target Selection Platform;
14）基因药物免疫治疗平台；
Gene Therapy Immunotherapy Platform;
15）变应原致敏性鉴定及分析平台；
Allergen Sensitization Identification and Analysis Platform;
16）变应原特异性免疫治疗药物开发平台；
Allergen-specific immunotherapy drug development platform;
17）上气道炎症性疾病发病机制研究与治疗靶点开发平台；
Research and Development Platform for the Pathogenesis and Therapeutic Targets of Upper Airway Inflammatory Diseases;
18）呼吸道上皮功能和分化检测平台；
Respiratory epithelial function and differentiation detection platform;
19）真皮再生模块的构建与功能化改进平台；
Platform for constructing and functional improvement of epidermal regeneration modules;
20）可移植界面材料涂层修饰平台等。
Portable interface material coating treatment for platforms, etc.
实验设备
experimental equipment
研究室拥有充足的仪器设备以维持多个技术平台的运行：如普通/高速/超高速/微型冷冻离心机、全自动细胞计数仪、CO2培养箱、生物安全柜、倒置荧光显微镜、高分辨激光共聚焦显微镜、活细胞成像分析仪、活细胞成像酶标仪、全视野细胞扫描分析仪、恒温金属浴、细菌摇床、脱色摇床、加热磁力搅拌器、制冰机、PCR仪、梯度PCR仪、实时荧光定量PCR仪、微滴式数字PCR仪、全自动密度梯度制备和分离系统、全自动智能蛋白液相色谱系统、全自动生化分析仪、冷冻干燥-真空离心浓缩系统、Octet分子互作仪、流式细胞仪、全自动磁性细胞分选仪、光谱流式细胞分析仪、组织研磨仪、程序降温仪、核酸定量测定仪、多功能酶标仪、微量核酸蛋白浓度定量仪、紫外切胶台、真空安全吸液系统等设备能够满足分子/细胞常规实验；以及多个低温设备能够满足临床生物样本库的建立；脑电图仪，声源定位仪，听觉脑干反应测试仪能满足神经听觉相关检测；同时体视显微镜、解剖显微镜、微量耳内注射系统能够满足更精细的动物实验。
The laboratory has sufficient instruments and equipment to support the operation of multiple technical platforms: such as normal/high-speed/ultra-high-speed/mini centrifuges, automatic cell counters, CO2 incubators, biological safety cabinets, inverted fluorescence microscopes, high-resolution laser confocal microscopes, live cell imaging analyzers, live cell imaging ELISA analyzers, full-field cell scanning analyzers, constant temperature metal baths, bacterial shakers, desorption shakers, heated magnetic stirrers, ice makers, PCR machines, gradient PCR machines, real-time fluorescence quantitative PCR machines, micro-droplet digital PCR machines, automatic density gradient preparation and separation systems, automatic intelligent protein liquid chromatography systems, automatic biochemical analyzers, freeze-drying-vacuum centrifugal concentration systems, Octet molecular interaction analyzers, flow cytometers, automatic magnetic cell sorting systems, spectral flow cytometry analyzers, tissue grinders, programmable cooling devices, nucleic acid quantification instruments, multi-function ELISA analyzers, micro-ultraviolet nucleic acid protein concentration quantifiers, gel cutting tables, vacuum safe liquid collection systems, etc. can meet the needs of molecular/cellular experiments; and multiple low-temperature equipment can meet the needs of the establishment of clinical biological sample banks; electroencephalography (EEG) instruments, sound source localization instruments, auditory brainstem response (ABR) test instruments can meet the needs of auditory neurophysiological testing; meanwhile, stereoscopic microscopes, anatomical microscopes, micro-injection systems for the ear can meet the needs of more delicate animal experiments.
常规开展技术
Conventional Technology
蛋白质检测技术：Western Blot蛋白表达与分离纯化、Elisa、CoIP、Chip等分子间相互作用技术；
Protein detection technologies: Western Blot protein expression and separation purification, ELISA, CoIP, Chip, etc. molecular interaction technologies;
核酸检测技术：Real-time PCR、微滴式数字PCR、DNA甲基化检测等；
Nucleic acid detection technology: Real-time PCR, droplet digital PCR, DNA methylation detection, etc.
分子克隆技术：过表达载体构建、CRISPR/Cas9载体构建、基因定点突变载体构建、慢病毒/腺病毒/腺相关病毒的包装等；
Molecular cloning techniques: construction of overexpression vectors, CRISPR/Cas9 vector construction, gene point mutation vector construction, packaging of lentivirus/adenovirus/adeno-associated virus, etc.
原代细胞培养与鉴定：上呼吸道肿瘤细胞、鼻黏膜原代细胞、毛细胞等细胞的原代分离培养等；
Primary cell culture and identification: isolation and culture of primary cells from respiratory tract tumors, nasal mucosa, cilia cells, etc.
细胞转染/病毒感染：脂质体转染、电转、慢病毒/腺病毒感染等；
Cell transfection/virus infection: liposome transfection, electroporation, lentivirus/adenovirus infection, etc.
细胞染色：免疫荧光、多重免疫荧光、细胞FDA/PI染色等；
Cell staining: immunofluorescence, multi-color immunofluorescence, cell FDA/PI staining, etc.
肿瘤细胞相关功能实验：肿瘤细胞迁移、侵袭、克隆形成、凋亡、周期等；
Tumor cell-related functional experiments: tumor cell migration, invasion, clonal formation, apoptosis, and cell cycle, etc.
细胞增殖检测：MTT、CCK-8、Edu检测和图像计数等；
Cell proliferation assay: MTT, CCK-8, Edu assay, and image counting, etc.
细胞培养：细胞复苏、细胞传代、细胞冻存、图像采集等；
Cell culture: cell revival, cell passage, cell cryopreservation, image acquisition, etc.
类器官培养：鼠及人正常上呼吸道黏膜、肿瘤组织、胆脂瘤类器官培养等；
Tissue Cultures: Normal upper respiratory mucosa of mouse and human, tumor tissue, cholesteatoma tissue cultures, etc.
流式细胞术：细胞凋亡/周期/自噬/磷酸化检测、免疫/肿瘤/干细胞等检测、磁珠分选、普通/无菌流式分选、外泌体检测等；
Flow cytometry: detection of apoptosis/cycle/autophagy/phosphorylation, immune/tumor/stem cell detection, magnetic bead sorting, general/sterile flow cytometry, exosome detection, etc.
动物听觉检测
Animal Hearing Detection
高分子递送系统：高分子载体设计与合成、质谱仪、核磁共振仪、红外光谱仪、凝胶渗透色谱仪、动态力学分析仪、热重分析仪和差示扫描量热仪等表征；
Polymer Delivery Systems: characterization of polymeric vectors using mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, gel permeation chromatography, dynamic mechanical analysis, thermogravimetric analysis, and differential scanning calorimetry, among others.
基因编辑检测：T7E1、TIDE、NGS deep sequencing等；
Gene editing detection: T7E1, TIDE, NGS deep sequencing, etc.
成像检测：奥林巴斯正置荧光显微镜、ZEISS全自动正置荧光显微镜 Apotome、Nikon激光共聚焦显微镜、奥林巴斯活细胞成像微孔板检测系统 Spin、病理大组织全切片扫描系统等；
Imaging detection: Olympus inverted fluorescence microscope, Zeiss fully automatic inverted fluorescence microscope Apotome, Nikon laser confocal microscope, Olympus live cell imaging microplate detection system Spin, and pathological whole tissue section scanning system, etc.
动物造模技术：顺铂及氨基糖甙类抗生素耳毒性动物模型构建，噪声性聋动物模型构建，条件性基因敲除小鼠动物模型构建，基因编辑斑马鱼模型构建及繁育，基因编辑爪蟾模型构建及繁育等;
Animal modeling techniques: construction of cisplatin and aminoglycoside antibiotic ototoxicity animal models, construction of noise-induced deafness animal models, construction of conditional gene knockout mouse animal models, construction and breeding of gene-edited zebrafish models, and construction and breeding of gene-edited Xenopus models.
动物听力、平衡与行为评估技术：小鼠、爪蟾等模式动物的ABR测听，小鼠平衡木及转棒实验等;
Techniques for assessing animal hearing, balance, and behavior: auditory brainstem response (ABR) hearing tests in mice and Xenopus laevis, and balance beam and bar rotations tests in mice.
电生理技术：毛细胞膜片钳实验;
Electrophysiological techniques: patch-clamp experiments on hair cell membranes;
内耳组织病理学评估技术：内耳解剖、毛细胞铺片、石蜡与冰冻组织包埋与切片、HE染色形态学评估、免疫组化、原位杂交、耳蜗及前庭细胞定量等;
Pathological assessment techniques for inner ear tissue: inner ear anatomy, hair cell smears, paraffin and frozen tissue embedding and sectioning, HE staining morphological assessment, immunohistochemistry, in situ hybridization, cochlear and vestibular cell quantification, etc.
内耳显微注射技术：鼓室内注射，鼓室后注射，卵圆窗注射，半规管注射等。
Microinjection techniques for inner ear: injection into the cochlea, injection into the cochlear foramen, injection into the oval window, and injection into the semicircular canals.
其他实验技术：细胞活性氧检测、自噬检测、线粒体提取及功能检测、线粒体能量代谢检测分析等。
Other experimental techniques: detection of reactive oxygen species in cells, autophagy detection, mitochondrial extraction and functional detection, mitochondrial energy metabolism detection analysis, etc.
数据挖掘：基因组、蛋白组、代谢组、bulk转录组、单细胞转录组、单细胞基因组、空间转录组、免疫组、表观组（包括甲基化、乙酰化、磷酸化等）、宏基因组、宏转录组、3D基因组、ATAC、HiC、Chip、Hi-Chip、时空代谢组学等单组学及多组学联合分析；以及队列人群GWAS、TWAS、PheWAS、孟德尔随机化、环境基因交互、PWAS、ExWAS、EpiWAS、meGWAS和microGWAS、群体遗传、演化基因组学等队列数据挖掘；人工智能算法开发等；
Data mining: genomics, proteomics, metabolomics, bulk transcriptomics, single-cell transcriptomics, single-cell genomics, spatial transcriptomics, immunomics, epigenetics (including methylation, acetylation, phosphorylation, etc.), metagenomics, metatranscriptomics, 3D genome, ATAC, HiC, Chip, Hi-Chip, temporal metabolomics, etc. single omics and multi-omics combined analysis; as well as cohort population GWAS, TWAS, PheWAS, Mendelian randomization, environmental gene interaction, PWAS, ExWAS, EpiWAS, meGWAS and microGWAS, population genetics, evolutionary genomics, etc. cohort data mining; development of artificial intelligence algorithms, etc.
三、队列及样本库建设
Cohort and Biobank Development
在科室领导、临床医疗组长、慢病随访办公室、华西生物样本库、临床研究管理部、老年医学中心、信息中心、华西生物医学大数据中心、深底医学中心的大力支持下，我科建立了头颈肿瘤慢病随访队列、老年性聋社区队列、助听干预队列，同时参与了华西医院10万例自然人队列的建设。本研究室构建了我科专病样本库及WCENT专病数据库，在场地、硬件、软件、人力方面进行长远布局，为我科战略性学科建设内容之一。数据库已实现入组患者全周期随访数据及样本智能化管理，可进行多中心、多人、多端口数据连载集成分析，集高效智能化数据采集、智能化质量控制、标准化研究设计、规范化数据分析等功能于一体，为我科临床及基础科研奠定坚实的数据及样本基础。
With the support of the department leadership, clinical medical team leaders, the chronic disease follow-up office, the Sichuan University West China Hospital Biological Sample Bank, the clinical research management department, the Center for Geriatric Medicine, the information center, the West China Biomedical Big Data Center, and the Shenbi Medical Center, our department has established a head and neck cancer chronic disease follow-up cohort, a community cohort for age-related hearing loss, and an intervention cohort for hearing aids. At the same time, our department has participated in the construction of the 100,000-case natural person cohort at West China Hospital. Our research center has built a special disease sample library and a WCENT special disease database, and has made long-term layouts in terms of venue, hardware, software, and manpower. It is one of the strategic academic construction content of our department. The database has realized the intelligent management of the full cycle of follow-up data and samples for enrolled patients, and can conduct serial and integrated analysis of multi-center, multi-person, and multi-port data. It integrates efficient and intelligent data collection, intelligent quality control, standardized research design, and standardized data analysis functions into one, laying a solid data and sample foundation for our department's clinical and basic research.
四、研究人员、研究方向简介
Overview of research staff and research focus
研究室主任：赵宇
Head of Research Lab: Yu Zhao
耳鼻咽喉头颈外科主任、听力中心主任、教授、博士生导师
Director of Otorhinolaryngology Head and Neck Surgery, Director of Hearing Center, Professor, Doctoral Advisor
研究方向检索词：耳聋；中耳炎；耳鸣；听力障碍；内耳疾病；老年性耳聋；遗传性耳聋；药物性耳聋；
Research Focus Keywords: hearing loss; middle ear infection; tinnitus; hearing impairment; inner ear disease; age-related hearing loss; hereditary hearing loss; drug-induced hearing loss;
研究方向:
Research Focus：
1) 大型自然人群队列数据挖掘，开展全基因组关联分析、共病遗传、环境基因交互、孟德尔随机化等研究；
Large-scale population-based cohort data mining, conducting genome-wide association studies, co-morbidity genetics, environmental gene interactions, and Mendelian randomization studies.
2) 围绕耳聋遗传，结合进行全基因组测序数据以及家系数据进行Townes-Brocks综合征(TBS)、CD164突变、Kcnq4点突变、Pendrin致病机制相关研究；
Conduct research on the inheritance of hearing loss, combining whole-genome sequencing data and pedigree mdata to study the Townes-Brocks Syndrome (TBS), CD164 mutations, Kcnq4 point mutations, and the pathogenic mechanisms of Pendrin.
3) 围绕内耳发育、药物性耳聋、胆脂瘤及中耳炎等开展相关机制研究。
Conduct research on the related mechanisms of inner ear development, drug-induced deafness, cholesteatoma, and middle ear infections.
研究室副主任：任建君
Deputy Director of the Research Center: Jianjun Ren
研究员、博士生导师
Researcher, PhD supervisor
研究方向检索词：头颈肿瘤、口咽癌、乳头状瘤、HPV、疫苗免疫、肿瘤新抗原、类器官、基因编辑、大数据挖掘
Research Focus Keywords: Head and Neck Tumors, Oral and Pharyngeal Cancer, Papilloma Virus, Vaccine Immunization, New Tumor Antigens, Organoids, Gene Editing, Big Data Mining
研究方向:
Research Focus：
1）大型自然人群队列数据挖掘，开展全基因组关联分析、共病遗传、环境基因交互、孟德尔随机化等研究；
Large-scale population-based cohort data mining, conducting genome-wide association studies, co-morbidity genetics, environmental gene interactions, and Mendelian randomization studies.
2）围绕HPV相关头颈肿瘤（包括良恶性肿瘤）开展了一系列临床及机制研究，围绕其免疫应答及调控、免疫微环境、治疗型肿瘤疫苗、肿瘤新抗原、肿瘤发生发展等领域，基于多组学数据分析、类器官模型、基因编辑、模式动物构建等技术，开展了深入研究。
A series of clinical and mechanistic studies have been conducted on HPV-related head and neck tumors (including benign and malignant tumors), focusing on immune responses and regulation, immune microenvironment, therapeutic tumor vaccines, novel tumor antigens, tumorigenesis and progression, etc. Based on multi-omics data analysis, organoid models, gene editing, and model animal construction, in-depth studies have been conducted.
研究室PI团队简介
Introduction to the PI Team of the Research Lab
（1） PI：刘世喜 耳鼻咽喉头颈外科学科主任、教授、博士生导师
PI:Shixi Liu, Academic Director of the Department of Otolaryngology, Head and Neck Surgery, Professor, PhD Supervisor
研究方向检索词：鼻窦炎，鼻息肉，过敏性鼻炎，鼻黏膜炎症
Research Focus Keywords:Nasal Sinusitis, Nasal Polyps, Allergic Rhinitis, Nasal Mucosal Inflammation
研究方向:
Research Focus：
主研鼻黏膜炎性疾病的病因、发病机制以及治疗策略，涉及鼻窦炎鼻息肉，过敏性鼻炎等鼻黏膜炎性疾病。目前开展了鼻窦炎鼻息肉的发病机制研究，包括非编码RNA在鼻窦炎鼻息肉中的作用机制研究，以及巨噬细胞极化在鼻窦炎鼻息肉中的作用及机制研究。
Specializing in the etiology, pathogenesis, and treatment strategies of nasal mucosal inflammatory diseases, including sinusitis and nasal polyps, as well as allergic rhinitis, etc. Currently, research on the pathogenesis of sinusitis and nasal polyps is being conducted, including the role and mechanism of non-coding RNA in sinusitis and nasal polyps, as well as the role and mechanism of macrophage polarization in sinusitis and nasal polyps.
（2） PI：邹剑 耳鼻咽喉头颈外科第一党支部书记兼副主任、教授、硕士生导师
PI: Jian Zou,Secretary of the First Party Branch and Deputy Director of the Department of Otorhinolaryngology, Head and Neck Surgery, Professor, Master's Supervisor
研究方向检索词：睡眠呼吸暂停、鼾症、间歇性低氧、微生物菌群、免疫失衡、喉癌、侵袭、转移
Research Focus Keywords: sleep apnea, snoring, intermittent hypoxia, microbial community, immune imbalance, laryngeal cancer, invasion, metastasis.
研究方向：
Research Focus：
1）成人阻塞性睡眠呼吸疾病（OSA）神经肌肉调控机制；
Neural and muscular regulation mechanisms of obstructive sleep apnea (OSA) in adults;
2）儿童OSA上气道微生物改变和免疫失衡的研究；
Research on the changes in upper airway microorganisms and immune imbalance in children with OSA;
3）喉癌的侵袭与转移机制。
The invasion and metastasis mechanism of laryngeal cancer.
（3） PI：杨慧 耳鼻咽喉头颈外科第二党支部书记兼副主任、教授、博士生导师
PI: Yang Hui, Secretary of the First Party Branch and Deputy Director of the Department of Otorhinolaryngology, Head and Neck Surgery，Professor, PhD Supervisor
研究方向检索词：咽喉，嗓音，头颈肿瘤
Research Focus Keywords: Pharynx, Voice, Head and Neck Tumors
研究方向：
Research Focus：
1）头颈部肿瘤的分子分型，靶点发现与个体化精准诊治
Molecular subtyping, target discovery, and individualized precision diagnosis and treatment for head and neck tumors.
2）T细胞治疗喉恶性肿瘤安全性和疗效性的临床研究
A clinical study on the safety and efficacy of T-cell therapy for malignant tumors of the larynx.
3）溶瘤病毒治疗实体瘤的临床研究
Clinical studies on oncolytic virus therapy for solid tumors
4）组织工程干细胞在声带损伤修复中的应用及机制研究
Application and mechanism of tissue engineering stem cells in repairing vocal cord injuries.
5）新型高分子材料在声带粘连中的防治及抗纤维化机制研究
Research on the Prevention and Anti-fibrosis Mechanism of Vocal Cord Adhesion Using New Polymer Materials
6）基于AI和深度学习的嗓音模型构建及其在疾病分类中的应用
Construction of voice model based on AI and deep learning and its application in disease classification.
（4） PI：郑芸 耳鼻咽喉头颈外科副主任、教授、博士生导师
PI: Yun Zheng, Associate Director of the Department of Otolaryngology, Head and Neck Surgery, Professor, PhD Supervisor
研究方向检索词：耳鸣、耳聋、眩晕、预防、治疗、生活方式医学、疗效评估、中西医结合
Research Focus Keywords:Tinnitus, Deafness, Vertigo, Prevention, Treatment, Lifestyle Medicine, Efficacy Assessment, Integration of Traditional Chinese Medicine and Western Medicine
研究方向：
Research Focus：
1）耳鸣耳聋眩晕中西医结合防治;
Integrative Chinese and Western Medicine Treatment for Tinnitus, Deafness, and Vertigo ;
2）生活方式医学与耳鸣耳聋眩晕防治;
Lifestyle Medicine and Prevention of Tinnitus, Deafness, and Vertigo ;
3）耳鸣耳聋眩晕疗效评估.
Evaluation of the effects of tinnitus, deafness, and dizziness.
（5） PI：周鹏 耳鼻咽喉头颈外科副主任、副教授
PI: Peng Zhou, Associate Director and Associate Professor of the Department of Otolaryngology, Head and Neck Surgery
研究方向检索词：鼻息肉、面神经、修复与重建、微小RNA。
Research Focus Keywords: nasal polyps, facial nerve, repair and reconstruction, microRNA.
研究方向：
Research Focus：
1）鼻息肉发病机制研究；
Research on the pathogenesis of nasal polyps;
2）面神经再生修复与重建；
Facial nerve regeneration and reconstruction;
3）面瘫及面部微表情识别（川大智胜共研）。
Facial Paralysis and Facial Micro-Expression Recognition (co-developed with Chengdu University of Intelligence and Technology).
（6） PI：孟娟 变态反应中心副主任、教授、硕士生导师
PI: Juan Meng,Associate Director of the Allergic Reaction Center, Professor, Master's Supervisor
研究方向检索词：鼻炎，鼻窦炎，气道验证，过敏原，过敏。
Research Focus Keywords: rhinitis, sinusitis, airway verification, allergens, allergies.
研究方向：
Research Focus：
1）上气道炎症性疾病（过敏性鼻炎、鼻窦炎）诊治及发病机制相关研究；
Upper Respiratory Inflammatory Diseases (Allergic Rhinitis, Sinusitis) Diagnosis and Treatment, as Well as Related Research on Pathogenesis;
2）其他过敏性疾病（食物过敏、药物过敏、严重过敏反应）诊治及发病机制相关研究。
Diagnosis and treatment of other allergic diseases (food allergies, drug allergies, severe allergic reactions) and related research on the pathogenesis.
（7） PI：孟照莉 听力中心副主任、教授、硕士生导师
PI: Zhaoli Meng, Associate Director of the Hearing Center, Professor, Master's Supervisor
研究方向检索词：听力学、老年性聋、耳鸣
Research Focus Keywords: audiology, age-related hearing loss, tinnitus
研究方向：
Research Focus：
1）老年性聋的特点、干预、发病机理
Characteristics, Intervention, and Pathogenesis of Presbycusis
2）耳鸣问卷表的设计
Design of the Tinnitus Questionnaire
（8） PI：陈飞 头颈外科疑难肿瘤中心主任、教授、硕士生导师
PI: Chen Fei, Head and Neck Surgery Center for Complex Tumors, Director, Professor, Master's Supervisor
研究方向检索词：头颈肿瘤；喉移植；气管移植
Research Focus Keywords:Head and Neck Tumors; Larynx Transplant; Trachea Transplant
研究方向：
Research Focus：
1）头颈肿瘤基础与临床研究；
Basic and Clinical Research on Head and Neck Tumors;
2）头颈部器官移植（以喉、气管、甲状腺移植为代表）。
Head and Neck Organ Transplants (represented by larynx, trachea, and thyroid gland transplants)
（9） PI：吕丹 华西天府医院耳鼻咽喉头颈外科主任、副教授、硕士生导师
PI: Dan Lu, Director of the Department of Otolaryngology, Head and Neck Surgery, Associate Professor, and Master's Supervisor at West China Hospital of Sichuan University
研究方向检索词：声门闭合不全；声带瘢痕；声带振动；聚氨酯；水凝胶；组织粘合剂；止血海绵
Research Focus Keywords: Glottal closure incompetence; vocal cord scarring; vocal cord vibration; polyurethane; hydrogel; tissue adhesive; hemostatic sponge
研究方向：
Research Focus：
1）高分子水凝胶在单侧声带麻痹/声带瘢痕/声带萎缩动物模型的应用；
Application of polymeric hydrogels in animal models of unilateral vocal cord paralysis/vocal cord scar/vocal cord atrophy ；
2）组织粘合剂在出血动物模型中的应用；
Application of tissue adhesives in bleeding animal models ；
3）止血海绵在出血动物模型中的应用。
Application of hemostatic sponges in bleeding animal models.
（10） PI：刘吉峰 华西天府医院耳鼻咽喉头颈外科副主任、副教授、硕士生导师
PI:Jifeng Liu, Deputy Director of the Ear, Nose, Throat and Head and Neck Surgery Department, Associate Professor, Master's Supervisor, West China Tianfu Hospital
研究方向检索词：极低本底辐射，头颈肿瘤，极端环境，高温
Research Focus Keywords:Extremely Low Background Radiation, Head and Neck Tumors, Extreme Environments, High Temperature
研究方向：头颈肿瘤与深地医学
Research Focus： Head and Neck Tumors and Deep Earth Medicine
（11） PI：唐玥玓 教授、博士生导师
PI: Professor Yuedi Tang, PhD supervisor
研究方向检索词：内耳临床基础研究
Research Focus Keywords: Clinical Basic Research on the Inner Ear
研究方向：血管纹；边缘细胞；外沟细胞；耳聋。
Research Focus： Vascular Ridges; Edge Cells; Outer Sulcus Cells; Deafness.
（12） PI ：刘锋 教授、硕士生导师
PI: Professor Feng Liu, Master's Supervisor
研究方向检索词：耳鼻咽喉科学，鼻科学，变态反应学
Research Focus Keywords: Otolaryngology, Rhinology, Allergology
研究方向：
Research Focus：
1）上呼吸道炎症疾病发病机制和临床研究;
Research on the pathogenesis and clinical studies of upper respiratory tract inflammatory diseases;
2）鼻腔鼻窦肿瘤发病机制和临床研究.
Research on the pathogenesis and clinical study of nasal and sinus tumors.
（13） PI：刘均 副教授、硕士生导师
PI: Associate Professor Jun Liu, Master's Supervisor
研究方向检索词：头颈肿瘤，修复重建，术前新辅，综合治疗，喉气管狭窄，高分子材料
Research Focus Keywords: Head and Neck Tumors, Reconstructive Surgery, Pre-operative New Assistants, Comprehensive Therapy, Laryngotracheal Stenosis, High Molecular Materials
研究方向：
Research Focus：
1）头颈肿瘤的外科手术治疗;
Surgical treatment for head and neck tumors;
2）晚期头颈肿瘤的术前新辅治疗;
New Adjuvant Therapy for Advanced Head and Neck Cancer;
3）晚期复发头颈肿瘤MDT治疗;
Multidisciplinary Treatment for Recurrent Head and Neck Cancer in Advanced Stages ;
4）喉气管狭窄临床及基础研究;
Clinical and Basic Research on Laryngotracheal Stenosis ;
5）喉气管狭窄相关高分子材料研究.
Research on High-Molecular Materials Related to Laryngotracheal Stenosis.
（15）PI：刘超 四川大学华西医院耳鼻喉头颈外科研究室 副研究员
PI:Chao Liu, Associate Researcher, Department of Otolaryngology-Head and Neck Surgery, West China Hospital, Sichuan University
研究方向检索词：基因编辑、递送系统、基因治疗
Research Focus Keywords: Gene Editing, Delivery Systems, Gene Therapy
研究方向：基于CRISPR/Cas9基因编辑技术的核酸药物设计与递送系统研究
Research Focus：Research on nucleic acid drug design and delivery system based on CRISPR/Cas9 gene editing technology.
研究室技术人员
Research Lab Technician
（1）数据分析师：舒涛、邵秀丽
Data Analysts:Tao Shu and Xiuli Shao
（2）实验技师：江传欢、梁艳玲、陈欣
Experimental Technicians: Chuanxuan Jiang, Yanling Liang, and Xin Chen
（3）科研助理：聂宏伟、侯宇、伍小燕、李蕤华、王泽艳、路大美
Research Assistants:Hongwei Nie,Yu Hou, Xiaoyan Wu,Ruihua Li , Zeyan Wang, Damai Lu
